Pre-lab Assignment 2

 

Name:                                                                                                                                                     Score:                     /5

 

Please prepare your answers on separate pages

 

1)      There are two popular methods for performing site directed mutagenesis of proteins.  Kunkel mutagenesis and QuickChange mutagenesis.  What are the similarities and differences between these techniques and why have we chosen to do Kunkel mutagenesis? 

 

 

 

2)      One step in the procedure involves transformation of bacteria using electroporation, what does this mean?

 

 

 

3)      The final step will be an overnight growth of the bacteria on LB-agar plates containing ampicillin, why?

 

 

 

 

 

 

 

 

Notebook assignment                                                                           Score:                          /5

 

Read the DNA chapter in the lab manual.  We will be working through the mutagenesis and electroporation procedures on Tuesday.  Outline these steps in your notebook.  Include any additional notes, thoughts or questions.

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