Pre-lab assignment 4

 

Name:                                                                                                                                                     Score:                     /5

 

Pages 296-298 from Lehninger Principles of Biochemistry may be helpful to answer the following questions.

 

 

1.  What is a ddNTP and what role does it play in DNA sequencing?

 

 

 

 

 

 

 

 

2.  Define what a primer is and verify that the T7 promoter sequence is appropriate for our plasmid (pET-21b from Novagen)

 

 

 

 

 

 

 

 

3.  We will be sending our plasmid DNA to Northwoods DNA Inc. (http://www.nwdna.com) for sequencing.  How much should we send, how should it be prepared, how should it be labeled and how much will this cost?

 

 

           

 

 

 

 

Notebook assignment (5 points)

 

Outline in your notebook the electrophoresis conditions for running a DNA sequencing gel.  Include gel size, gel composition, power requirements, length of time and sample loading parameters (how much?).

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