Pre-lab assignment 5


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The chemical method for cell lysis utilizes a zwitterionic detergent that disrupts cell membranes.  Propose a mechanism for how this process works.  What are the advantages?
















Draw the structure of imidazole, what does it do in this experiment?











Many of the buffers contain b-mercaptoethanol.  What is its role in this purification procedure?





Notebook assignment                                                                                                               /5


Outline the experimental steps needed to isolate your wild type and mutant CBL from the provided bacterial culture.  Include any thoughts about controls and questions you may generate.