Pre-lab assignment 5
Name: Score: /5
The chemical method for cell lysis utilizes a zwitterionic detergent that disrupts cell membranes. Propose a mechanism for how this process works. What are the advantages?
Draw the structure of imidazole, what does it do in this experiment?
Many of the buffers contain b-mercaptoethanol. What is its role in this purification procedure?
Notebook assignment /5
Outline the experimental steps needed to isolate your wild type and mutant CBL from the provided bacterial culture. Include any thoughts about controls and questions you may generate.