Pre-lab assignment 5

 

Name:                                                                                                                                                     Score:                     /5

 

The chemical method for cell lysis utilizes a zwitterionic detergent that disrupts cell membranes.  Propose a mechanism for how this process works.  What are the advantages?

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Draw the structure of imidazole, what does it do in this experiment?

 

 

 

 

 

 

 

 

 

 

Many of the buffers contain b-mercaptoethanol.  What is its role in this purification procedure?

 

 

 

 

Notebook assignment                                                                                                               /5

 

Outline the experimental steps needed to isolate your wild type and mutant CBL from the provided bacterial culture.  Include any thoughts about controls and questions you may generate.