Pre-lab assignment 6
Name: Score: /5
1. The extinction coefficient for a hypothetical protein is 1.4x105 AU M-1cm-1. How is this value used to determine concentration of the protein?
2. You have hypothetically isolated a pure sample of carbonic anhydrase with a concentration of 43 mcM (micromolar can be abbreviated as mcM or mM). What is the concentration in mg/mL?
3. Show the calculations for preparing 1.0 mL of three bovine serum albumin (BSA) solutions in water at the following concentrations given a stock solution of BSA at 2.00 mg/mL?
Notebook assignment /5
Using the lab manual and any other necessary resources, decide whether the BCA assay or the Bradford assay is best for determining the concentration of cystathionine b-lyase. Include any procedural notes you may need to conduct the assay.