Pre-lab assignment 7


Name:                                                                                                                                                     Score:                     /5


1.  What percentage SDS-acrylamide gel is appropriate for identifying a protein with a molecular weight of 45kD?






2.  What are the two properties of SDS that make it important in this experiment?









3. What is the minimum amount of protein that must be present in a protein band on an SDS-acrylamide gel for it to be visualized using Coomassie brilliant blue as the stain?











Notebook assignment.  Score                           /5.  Review the procedure for SDS-PAGE.  Given your calculated concentration of CBL and mutant CBL from last week, show in your notebook the appropriate calculations to load the correct amount of protein/lane on a gel (based on your answer to question 3 above)