Pre-lab assignment 9

 

Name:                                                                                                                                                     Score:                     /5

 

 

 

1.  Based on the chemical mechanism of CBL hydrolysis and the crystal structure of CBL, what would you predict would happen to the Vmax of the reaction when cystathionine and djenkolic acid are used as substrates? 

 

 

 

 

 

 

 

 

 

2.  Based on the chemical mechanism of CBL hydrolysis and the crystal structure of CBL, what would you predict would happen to the Km of the reaction when cystathionine and djenkolic acid are used as substrates?

 

 

 

 

 

 

 

3.  Revisit your hypothesis with regard to the CBL mutation you designed.  What would you expect to happen with the various substrates with respect to Km and Vmax?

 

 

 

 

Notebook assignment                                       /5  Outline an experiment to support your answers for 1-3 above.  Be as specific in your design as possible as you will be conducting this experiment on Tuesday!  Include thoughts on how the graphically display the data.

 

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